Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves.
Identifieur interne : 000110 ( Main/Exploration ); précédent : 000109; suivant : 000111Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves.
Auteurs : Misa Takahashi [Japon] ; Jun Shigeto [Japon] ; Shunsuke Izumi [Japon] ; Katsutoshi Yoshizato [Japon] ; Hiromichi Morikawa [Japon]Source :
- Plant signaling & behavior [ 1559-2324 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Feuilles de plante, Protéines végétales, Tabac.
- métabolisme : Dioxyde d'azote, Feuilles de plante, Nitrates, Protéines végétales, Tabac, Vacuoles.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Nitrates, Nitrogen Dioxide, Plant Proteins.
- genetics : Plant Leaves, Tobacco.
- metabolism : Plant Leaves, Tobacco, Vacuoles.
Abstract
Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr(36) of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.
DOI: 10.1080/15592324.2016.1197464
PubMed: 27301959
PubMed Central: PMC4991344
Affiliations:
Links toward previous steps (curation, corpus...)
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xml:lang="fr">Japon</country><wicri:regionArea>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima </wicri:regionArea><wicri:noRegion>Higashi-Hiroshima </wicri:noRegion></affiliation></author><author><name sortKey="Shigeto, Jun" sort="Shigeto, Jun" uniqKey="Shigeto J" first="Jun" last="Shigeto">Jun Shigeto</name><affiliation wicri:level="1"><nlm:affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima </wicri:regionArea><wicri:noRegion>Higashi-Hiroshima </wicri:noRegion></affiliation></author><author><name sortKey="Izumi, Shunsuke" sort="Izumi, Shunsuke" uniqKey="Izumi S" first="Shunsuke" last="Izumi">Shunsuke Izumi</name><affiliation wicri:level="1"><nlm:affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima </wicri:regionArea><wicri:noRegion>Higashi-Hiroshima </wicri:noRegion></affiliation></author><author><name sortKey="Yoshizato, Katsutoshi" sort="Yoshizato, Katsutoshi" uniqKey="Yoshizato K" first="Katsutoshi" last="Yoshizato">Katsutoshi Yoshizato</name><affiliation wicri:level="1"><nlm:affiliation>b Department of Biological Science , Hiroshima University, Higashi-Hiroshima , Hiroshima , Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>b Department of Biological Science , Hiroshima University, Higashi-Hiroshima , Hiroshima </wicri:regionArea><wicri:noRegion>Hiroshima </wicri:noRegion></affiliation></author><author><name sortKey="Morikawa, Hiromichi" sort="Morikawa, Hiromichi" uniqKey="Morikawa H" first="Hiromichi" last="Morikawa">Hiromichi Morikawa</name><affiliation wicri:level="1"><nlm:affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima </wicri:regionArea><wicri:noRegion>Higashi-Hiroshima </wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant signaling & behavior</title><idno type="eISSN">1559-2324</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Nitrates (metabolism)</term><term>Nitrogen Dioxide (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Vacuoles (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dioxyde d'azote (métabolisme)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Nitrates (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Vacuoles (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrates</term><term>Nitrogen Dioxide</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Protéines végétales</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term><term>Vacuoles</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dioxyde d'azote</term><term>Feuilles de plante</term><term>Nitrates</term><term>Protéines végétales</term><term>Tabac</term><term>Vacuoles</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr(36) of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27301959</PMID><DateCompleted><Year>2017</Year><Month>10</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1559-2324</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>07</Month><Day>02</Day></PubDate></JournalIssue><Title>Plant signaling & behavior</Title><ISOAbbreviation>Plant Signal Behav</ISOAbbreviation></Journal><ArticleTitle>Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves.</ArticleTitle><Pagination><MedlinePgn>e1197464</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1080/15592324.2016.1197464</ELocationID><Abstract><AbstractText>Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr(36) of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Takahashi</LastName><ForeName>Misa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shigeto</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Izumi</LastName><ForeName>Shunsuke</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yoshizato</LastName><ForeName>Katsutoshi</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>b Department of Biological Science , Hiroshima University, Higashi-Hiroshima , Hiroshima , Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morikawa</LastName><ForeName>Hiromichi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>a Department of Mathematical and Life Sciences , Hiroshima University , Higashi-Hiroshima , Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Signal Behav</MedlineTA><NlmUniqueID>101291431</NlmUniqueID><ISSNLinking>1559-2316</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>S7G510RUBH</RegistryNumber><NameOfSubstance UI="D009585">Nitrogen Dioxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009585" MajorTopicYN="N">Nitrogen Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014617" MajorTopicYN="N">Vacuoles</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Apoplast</Keyword><Keyword MajorTopicYN="Y">PR-1</Keyword><Keyword MajorTopicYN="Y">PR-3</Keyword><Keyword MajorTopicYN="Y">PR-5</Keyword><Keyword MajorTopicYN="Y">defense/immunity-related proteins</Keyword><Keyword MajorTopicYN="Y">nitrogen dioxide</Keyword><Keyword MajorTopicYN="Y">pathogenesis-related proteins</Keyword><Keyword MajorTopicYN="Y">protein tyrosine nitration</Keyword><Keyword MajorTopicYN="Y">tobacco</Keyword><Keyword MajorTopicYN="Y">vacuole</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>10</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27301959</ArticleId><ArticleId IdType="doi">10.1080/15592324.2016.1197464</ArticleId><ArticleId 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last="Yoshizato">Katsutoshi Yoshizato</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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